Biopython MCQs and Answers with Explanation: Don’t know the concept of Biopython and you are here to learn about it and at the same time to test your knowledge about it? then this Biopython Online Quiz can be a huge help to you. Biopython is a Python library designed for bioinformatics research and development. It provides a range of tools for working with biological data, including DNA and protein sequences, and offers a wide range of functionality for tasks like sequence alignment, protein structure analysis, and phylogenetic tree construction. Biopython is an open-source library and is widely used in the bioinformatics community. After understanding Biopython you should take a look at these MCQs on Biopython and can prepare for the interview or placement test to score well in it.
Biopython Multiple Choice Questions
In this article, we have accommodated some Biopython Multiple Choice Questions and their answers to help you test your knowledge of this powerful library. This Biopython Quiz covers a range of topics, from basic concepts like reading and writing sequence data to more advanced topics like phylogenetic analysis and protein structure prediction. Whether you’re new to Biopython or an experienced user looking to test your knowledge, these MCQs on Biopython will surely help you learn new things about Biopython easily.
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Biopython MCQ with Answers
| Name | Biopython |
| Exam Type | MCQ (Multiple Choice Questions) |
| Category | Technical Quiz |
| Mode of Quiz | Online |
Top 25 MCQs on Biopython | Biopython Quiz
1. What is Biopython?
a) A programming language
b) A library for biological data analysis
c) A database of biological sequences
d) A tool for gene editing
Answer: B A library for biological data analysis.
Explanation: Biopython is a Python library that provides tools for working with biological data.
2. What is the primary data structure used in Biopython?
a) Lists
b) Tuples
c) Dictionaries
d) Sets
Answer: C Dictionaries.
Explanation: Dictionaries are the primary data structure used in Biopython, as they allow for easy manipulation of data with key-value pairs.
3. Which Biopython module is used for sequence analysis?
a) SeqIO
b) AlignIO
c) Bio.Align
d) Bio.Seq
Answer: D Bio.Seq.
Explanation: The Bio.Seq module is used for sequence analysis in Biopython, including reading and writing sequences, translating DNA to protein, and performing various sequence manipulations.
4. Which Biopython module is used for sequence alignment?
a) SeqIO
b) AlignIO
c) Bio.Align
d) Bio.Seq
Answer: C Bio.Align.
Explanation: The Bio.Align module is used for sequence alignment in Biopython, including performing pairwise and multiple sequence alignments and creating consensus sequences.
5. Which Biopython module is used for phylogenetic analysis?
a) Phylo
b) ClustalW
c) Bio.Align
d) Bio.SeqIO
Answer: A Phylo.
Explanation: The Phylo module is used for phylogenetic analysis in Biopython, including constructing and manipulating phylogenetic trees.
6. What is the function of the SeqRecord object in Biopython?
a) To store a DNA sequence
b) To store a protein sequence
c) To store sequence annotations and metadata
d) To store a multiple sequence alignment
Answer: C To store sequence annotations and metadata.
Explanation: The SeqRecord object in Biopython is used to store a sequence along with annotations and metadata, such as the sequence ID, description, and source organism.
7. Which Biopython module is used for working with protein structures?
a) PDB
b) SeqIO
c) AlignIO
d) Bio.Align
Answer: A PDB.
Explanation: The PDB module in Biopython is used for working with protein structures, including reading and writing PDB files and performing various structure analysis and manipulation tasks.
8. What is the function of the Seq object in Biopython?
a) To store a DNA sequence
b) To store a protein sequence
c) To store sequence annotations and metadata
d) To store a multiple sequence alignment
Answer: A To store a DNA sequence. The Seq object in Biopython is used to store a DNA or RNA sequence.
9. Which Biopython module is used for working with BLAST?
a) BLAST
b) SeqIO
c) AlignIO
d) Bio.Blast
Answer: D Bio.Blast.
Explanation: The Bio.Blast module in Biopython is used for working with BLAST, including performing sequence searches and parsing BLAST output.
10. What is the purpose of the Bio.Alphabet module in Biopython?
a) To define a set of standard alphabets for biological sequences
b) To provide tools for working with DNA sequence data
c) To provide tools for working with protein sequence data
d) To provide tools for working with RNA sequence data
Answer: A To define a set of standard alphabets for biological sequences
Explanation:. The Bio.Alphabet module in Biopython defines a set of standard alphabets for working with biological sequence data, including DNA, RNA, and protein sequences.
11. Which Biopython module is used for working with Entrez?
a) SeqIO
b) Bio.Entrez
c) AlignIO
d) Bio.Align
Answer: B Bio.Entrez. The Bio.Entrez module in Biopython is used for accessing and retrieving data from the NCBI Entrez database, including retrieving sequences and performing searches.
12. Which method is used to translate DNA sequences to protein sequences in Biopython?
a) transcribe()
b) reverse_complement()
c) translate()
d) complement()
Answer: C translate(). The translate() method in Biopython is used to translate a DNA sequence to a protein sequence using the standard genetic code.
13. Which method is used to perform a reverse complement of a DNA sequence in Biopython?
a) transcribe()
b) reverse_complement()
c) translate()
d) complement()
Answer: B reverse_complement().
Explanation: The reverse_complement() method in Biopython is used to perform a reverse complement of a DNA sequence, swapping A and T, and C and G.
14. Which method is used to find the GC content of a DNA sequence in Biopython?
a) gc_content()
b) count()
c) find()
d) index()
Answer: A gc_content().
Explanation: The gc_content() method in Biopython is used to find the GC content of a DNA sequence, calculated as the number of G and C bases divided by the total sequence length.
15. Which method is used to perform a multiple sequence alignment in Biopython?
a) pairwise2()
b) align()
c) align_multiple()
d) alignSeqs()
Answer: C align_multiple().
Explanation: The align_multiple() method in Biopython is used to perform a multiple sequence alignment of a set of sequences, using a specified alignment algorithm.
16. Which method is used to read a FASTA file in Biopython?
a) read_fasta()
b) read()
c) parse()
d) readfasta()
Answer: C parse().
Explanation: The parse() method in Biopython is used to read a FASTA file and return a SeqRecord object.
17. Which method is used to write a FASTA file in Biopython?
a) write_fasta()
b) write()
c) format()
d) writefasta()
Answer: B write().
Explanation: The write() method in Biopython is used to write a SeqRecord object to a FASTA file.
18. Which module is used to calculate molecular weight in Biopython?
a) SeqUtils
b) SeqIO
c) AlignIO
d) Bio.Align
Answer: A SeqUtils.
Explanation: The SeqUtils module in Biopython is used to calculate various properties of a biological sequence, including molecular weight, isoelectric point, and codon usage.
19. Which module is used to calculate a consensus sequence in Biopython?
a) Bio.Align
b) SeqIO
c) SeqUtils
d) PDB
Answer: A Bio.Align.
Explanation: The Bio.Align module in Biopython is used to calculate a consensus sequence from a set of aligned sequences, based on the most common nucleotide or amino acid at each position.
20. Which method is used to perform a BLAST search in Biopython?
a) blast()
b) blastn()
c) blastp()
d) blastx()
Answer: B blastn().
Explanation: The blastn() method in Biopython is used to perform a nucleotide sequence search using the BLAST algorithm.
21. Which method is used to parse a BLAST XML output file in Biopython?
a) parse_blast()
b) read_blast()
c) parse()
d) read()
Answer: C parse().
Explanation: The parse() method in Biopython is used to parse a BLAST XML output file
22. Which method is used to retrieve a specific feature from a GenBank file in Biopython?
a) get_feature()
b) get_record()
c) get_seq()
d) get_sequence()
Answer: A get_feature().
Explanation: The get_feature() method in Biopython is used to retrieve a specific feature from a GenBank file, such as a coding sequence, exon, or promoter region.
23. Which module is used for handling protein structures in Biopython?
a) SeqIO
b) PDB
c) SeqUtils
d) AlignIO
Answer: B PDB.
Explanation: The PDB module in Biopython is used for working with protein structures, including reading and writing PDB files, manipulating structures, and performing structure-based calculations.
24. Which method is used to calculate the distance between two atoms in a protein structure in Biopython?
a) calc_angle()
b) calc_dihedral()
c) calc_distance()
d) calc_rmsd()
Answer: C calc_distance().
Explanation: The calc_distance() method in the PDB module of Biopython is used to calculate the distance between two atoms in a protein structure, based on their coordinates.
25. Which method is used to perform a structural superposition of two protein structures in Biopython?
a) align()
b) superimpose()
c) overlay()
d) match()
Answer: B superimpose().
Explanation: The superimpose() method in the PDB module of Biopython is used to perform a structural superposition of two protein structures, aligning them based on their common atoms and minimizing their root-mean-square deviation (RMSD).
We hope that this set of Biopython MCQ with Answers has provided you with a better understanding of Biopython, its features, and its applications. By testing your knowledge through these Biopython Multiple Choice Questions, you can gauge your level of understanding and identify areas for further improvement. Keep learning and exploring the world of Biopython, and don’t forget to visit Freshersnow to enhance your career.